Nucleic acid extraction from a periapical lesion without a background of endodontics: case report
DOI:
https://doi.org/10.15332/us.v15i0.2081Keywords:
Periapical granuloma, DNA, electrophoresisAbstract
Objective: the purpose of this in vitro study was to extract DNA from a periapical lesion and amplify a fragment of DNA from the bacterial 16s ribosomal subunit (DNAr 16s) using the Polymerase Chain Reaction (PCR) and electrophoresis.
Materials and methods: a sample obtained from a patient, the technique silica columns for DNA extraction was applied was analyzed. Subsequently the sample was processed with QIAamp® applying the corresponding protocol to confirm the bacteria presence by polymerase chain reaction (PCR) and electrophoresis.
Results: the volume of DNA obtained was 61 ng / uL purity 1.96. The electrophoresis results were negative for bacterial DNA. Which allowed to conclude that bacteria were not transferred to the peri-penice mechanically, since the sample was obtained from a molar that had no previous history of endodontics.
Conclusion: this result showed that although the specificity and sensitivity of the technique used, periapical lesions may also be sterile with the presence of healing tissue, which can be explained by the action of host macrophages as a defense mechanism in absence of pathogens.
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