IN VITRO ASSESSMENT OF ANTIMICROBIAN ACTIVITY OF CALCIUM HYDROXIDE IN TWO PREPARATIONS IN PRESENCE OF ENTEROCOCCUS FAECALIS
DOI:
https://doi.org/10.15332/us.v3i2.1876Keywords:
Calcium hydroxide, Chlorhexidine gluconate, Propylenglycol, Enterococcus faecalisAbstract
Objective: To assess the antimicrobial effect of two different mixes of calcium hydroxide: with propylenglycol and with chlorhexidine gluconate on Enterococcus faecalis (ATCC29212).
Material and methods: Through an experimental in vitro study two agents were tested: pure calcium hydroxide, calcium hydroxide with 13% chlorhexidine gluconate, and calcium hydroxide with propylenglycol. E. faecalis dilutions at 1.5 x 10-7 and 1.5 x 10-8 in soy trypticase were used, to which 100 mg. of each agent were added. They were cultured in Agar Mueller Hinton medium at these intervals: 1, 20, 60, 80, and 120 minutes in direct contact with E. Faecalis strain. They were then incubated at 37° Centigrades, to observe Colony Forming Units (CFU) at 24, 48, and 72 hours. Culture readings were conducted under supervision of one sole operator.
Results: Some CFU's were found after one minute of direct contact between microorganism and pure calcium hydroxide, in contrast with other study groups whose CFU readings were zero after one-minute contact. Chlorhexidine gluconate used at 13% showed a better performance; however, results showed no statistically significant differences.
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