Formulation of a culture medium for the production of δ-endotoxines of Bacillus thuringiensis
DOI:
https://doi.org/10.15332/us.v17i0.2185Keywords:
Bacillus thuringiensis, δ-endotoxins, culture medium, Cry proteinsAbstract
Objective: To perform the formulation of a culture medium for the production of parasporal proteins of Bacillus thuringiensis.
Methods: Five culture media were evaluated, contrasting their formulation with the production of biomass.
Results: The total protein was extracted from each of the cultures and quantified by spectrophotometry.
Conclusion: It was found that the glycoside broth modified with 2.4:1 proportions of organic (yeast extract) and inorganic ((NH4) 2SO4) nitrogen sources improves up to 70 times the production of protein with concentrations between 19.38 and 154, 41 mg / mL. This study contributes to the affirmation that the ratio between Organic and Inorganic Nitrogen have important effects on the phase III of the growth of Bacillus thuringiensis, contrasting with the production of endospores and consequently total proteins in which the increase in δ-endotoxins are included.
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