IN VITRO EVALUATION OF THE EFFECTIVENESS OF CHLORHEXIDINE DIGLUCONATE GEL 0.2% AND 2% AS AN INTRACANAL MEDICATION AGAINST CANDIDA ALBICANS
DOI:
https://doi.org/10.15332/us.v12i2.1211Keywords:
Candida albicans, Chlorhexidine digluconate, Antimicrobial activityAbstract
Objective: to assess the efficacy of chlorhexidine digluconate gel 0.2% and 2% used as intracanal medicaments against Candida albicans in roots of extracted human teeth.
Methods: an experimental In vitro study was done. The sample consisted in 48 single-rooted roots, which were instrumented with conventional technique to No. 40 K file and then they were inoculated with Candida albicans ATCC60193 for 72 hours. The samples were divided into three groups of 16 specimens each for intracanal medication with chlorhexidine digluconate 2% chlorhexidine digluconate 0.2% and 0.9% saline solution for seven days. After that each specimen was instrumented again with K-file number 40 to obtain the smear layer of the root canal system to be cultivated in 2% agar Sabouraud for 72 hours at 37 °C to determine the Count Forming Units per mL (CFU/mL) of Candida albicans.
Results: the model of this study allowed to establish that Candida albicans grew in all the root canals of the teeth before before the intracanal medication was injected. It was found that group three (treated with 0.9% saline solution) showed a statically significant difference with respect to the other two groups, chlorhexidine 2% and clorhexidine 0.2%. The Mann-Whitney U test also showed no significant differences in the CFU/mL in the two clorhexidine treated groups, the one with 0,2% and the one with 2%.
Conclusion: chlorhexidine digluconate gel 0.2% and 2%, as intracanal medication, significantly decreased CFU/mL of Candida albicans, but is not completely destroyed after a period of seven days.
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