Iron effect on growth and lipid accumulation in the colombian microalga Chlorella Vulgaris LAUN 0019
Abstract
En este trabajo se evalúa el efecto del ión ferroso (Fe2+) sobre el crecimiento y acumulación de lípidos totales de la microalga Chlorella vulgaris. Se empleó medio Bristol estándar para su cultivo; la cinética de crecimiento se midió por conteo directo y la determinación de lípidos totales se realizó mediante extracción con Soxhlet. Se estudiaron cinco diferentes concentraciones de este ión, entre 2,16 μM y 50,0 μM. El medio enriquecido con una concentración de 10,0 μM produjo la máxima velocidad específica de crecimiento celular (0,76 día-1), mientras que las máximas productividades de biomasa y de lípidos se presentaron a la concentración 5,00 μM con valores de 112,4 mg·L-1·día-1 y 6,52 mg·L-1·día-1 respectivamente. Para las concentraciones más altas de hierro (21,5 y 50,0 μM), la microalga presentó inhibición por sustrato. Finalmente, para concentraciones menores que 10,0 μM se encontró que para una significancia del 5% la concentración del hierro no afecta significativamente la productividad de biomasa y lípidos.
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References
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[3] C. M. Drapcho, N. P. Nhuan, and T. H. Walker, Biofuels Engineering Process Technology, vol. I., New York: McGraw Hill, 2008
[4] Fedepalma, “Oferta y consumo aparente mundial de los 17 principales aceites y grasas”. http://www.fedepalma.org/documen/2008/ consumo_mundial.pdf consultado 3 de Octubre de 2009
[5] FAO. “Food and Agricultural commodities production”, http:// faostat.fao.org/site/339/default. aspx consultado el Noviembre 06, 2010
[6] E. P. Feofilova, Y. E. Sergeeva, and A.A. Ivashechkin, “Biodiesel-fuel: Content, production, producers, contemporary biotechnology (Review)”, Applied Biochemistry and Microbiology, vol. 46, no. 4, pp. 369-378, 2010
[7] Y. Chisti, “Biodiesel from microalgae”, Biotechnology Advances, vol. 25, no. 3, pp. 294-306, 2007
[8] A. Banerjee, R. Sharma and Y. Chisti, “Botryococcus braunii: A Renewable Source of Hydrocarbons and Other Chemicals”, Critical Reviews in Biotechnology, vol. 22, no. 3, pp. 245-279, 2002
[9] G.Petkov and G.Garcia, “Which are fatty acids of the green alga Chlorella?”, Biochemical Systematics and Ecology, vol. 35, no. 5, pp. 281-285, 2007
[10] Acosta,. “Biocombustibles: Oportunidad o Amenaza”. Presented at the Seminario Internacional De Biocombustibles 2009 CORPODIB. Bogotá, Colombia, Febrero 12, 2009
[11] Z. Y. Liu, G. C. Wang and B. C. Zhou, “Effect of iron on growth and lipid accumulation in Chlorella vulgaris”, Bioresource Technology, vol. 99, no. 11, pp. 4717-4722, 2008
[12] R. Andersen, Algal culturing techniques, vol. I. China: Elsevier Inc., 2005
[13] F. Cleber Bertoldi, E. Sant’Anna, M. V. da Costa Braga and J. L. Barcelos Oliveira, “Lipids, fatty acids composition and carotenoids of Chlorella vulgaris cultivated”, Grasas y Aceites, vol. 57, no.3, pp. 270-274, 2006
[14] M. S. Estevez, G. Malanga and S. Puntarulo. “Iron-dependent oxidative stress in Chlorella vulgaris”, Plant Science, vol. 161, no.1, pp. 9-17, 2001.
[15] C. Yeesang and B. Cheirsilp, “Effect of nitrogen, salt, and iron content in the growth medium and light intensity on lipid production by microalgae isolated from freshwater sources in Thailand”, Bioresource Technology, vol. 102, no.3, pp. 3034-3040, 2011
[16] C. Zhuo-Ping, H. Wei-Wei, A. Min and D. ShunShan, “Coupled effects of irradiance and iron on the growth of a harmful algal bloom-causing microalga Scrippsiella trochoidea”, Acta Ecologica Sinica, vol. 29, no.5, pp. 297-301, 2009
[17] H. He, F. Chen, H. Li, W. Xiang, Y. Li and Y. Jiang, “Effect of iron on growth, biochemical composition and paralytic shellfish poisoning toxins production of Alexandrium tamarense”, Harmful Algae, vol. 9, no.1, pp. 98-104, 2010
[18] M. Cai, A. Qi, Y. Zhang and Z. Li, “Effects of iron electrovalence and species on growth of Haematococcus pluvialis and astaxanthin production”, Journal of Biotechnology, vol. 136, no.1, pp. S574, 2008
[19] M. Chen, H. Tang, H. Ma, T. C. Holland, K.Y. Simon Ng and S. O. Salley, “Effect of nutrients on growth and lipid accumulation in the green algae Dunaliella tertiolecta”, Bioresource Technology, vol. 102, no.2, pp. 1649-1655, 2011
[20] ASTM International. ASTM C 613/C 613M – 97. Standard Test Method for Constituent Content of Composite Prepreg by Soxhlet Extraction, 2008
[21] S. H. Cho, S.C. Ji, S. B. Hur, J. Bae, I.S. Park, Y. C. Song, “Optimum temperature and salinity conditions for growth of green algae Chlorella ellipsoidea and Nannochloris oculata”, Fisheries Science, vol. 73, no.5, pp. 1050–1056, 2007
[22] A. Ranga Rao, C. Dayananda, R. Sarada, T.R. Shamala and G.A. Ravishankar, “Effect of salinity on growth of green alga Botryococcus braunii and its constituents”, Bioresource Technology, vol. 98, no.3, pp. 560-564, 2007
Published
2011-06-30
How to Cite
Serrano Bermúdez, L., Ramírez Landínez, D., Sierra Sáenz, E., Scott Carvajal, O., Álvarez Sierra, C., Torres Parra, J., Narváez Rincón, P., & Godoy Silva, R. (2011). Iron effect on growth and lipid accumulation in the colombian microalga Chlorella Vulgaris LAUN 0019. ITECKNE, 8(1), 15-22. https://doi.org/https://doi.org/10.15332/iteckne.v8i1.257
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Section
Research and Innovation Articles
