Silver nanostructured platforms for detecting Escherichia coli through Raman scattering spectroscopy “Proof of concept”
Keywords:
Silver nanostructured platforms, SERS, bacteria, nanotechnology
Abstract
Surface enhanced Raman spectroscopy (SERS) of nanostructured materials is a powerful technique that allows to reach ultrahigh levels of detection of several analytes. In the present study it was possible to identify the bacteria E. coli by using a novel nanostructured platform based on silver-capped nanopillars (AgNP) and SERS technique. AgNPs were fabricated by ion reactive etching and deposition of silver layers. The bacteria culture were prepared in Luria-Bertani (LB) medium at 37° by 4 hours. 5 μL of the bacteria were deposited on top of the surface of AgNPs and let it dry for 30 minutes. Subsequently the AgNP-bacteria system was analysed by Raman spectroscopy. A typical band of E. coli at 731 cm-1 was identified and this Raman vibration was used as a marker peak to detect the bacteria. Finally the novel AgNP could be used as a potential biosensor to detect nosocomial bacteria in intra-hospital environments.Downloads
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References
M.S. Schmidt, J. Hübner, A. Boisen, “Large Area Fabrication of Leaning Silicon Nanopillars for Surface Enhanced Raman Spectroscopy”, Adv Mat; 10, pp. 11-18, 2011.
A.J. Driscoll, M.H. Harpster, P.A. Johnson, “The development of surface-enhanced Raman scattering as a detection modality for portable in vitro diagnostics: progress and challenges”, Phys Chem Phys Chem 2013; 15, pp. 20415-20433. doi: 10.1039/c3cp52334a
W.R. Premasiri, N. Krieger, W.R. Associates, “Characterization of the Surface Enhanced Raman Scattering (SERS) of Bacteria”, J Chem Phys B; 109, pp. 312-320, 2005.
J. Chen, X. Wu, Y. Huang, Y. Zhao, “Chemical Detection of E. coli using SERS active filters with silver nanorod array”, Sensors Actuators B Chem; 191, pp. 485-490, 2014
J.J. Castillo, T. Rindzevicius, K. Wu et al. “Silver-capped silicon nanopillar platforms for adsorption studies of folic acid using surface enhanced Raman spectroscopy and density functional theory”, J Raman Spect; 46, pp. 1087-1094, 2015.
J.J. Castillo, T. Rindzevicius et al., “Adsorption and Vibrational Study of Folic Acid on Gold Nanopillar Structures Using Surface-enhanced Raman Scattering Spectroscopy Regular Paper”, Nanomat Nanotechn; 5: 1-7, 2015.
J. Borrero, Y. Chen, G. Dunny, Y. Kaznessis, “Modified Lactic Acid Bacteria Detect and Inhibit Multiresistant Enterococci”, ACS Synthet. Biol.; 4, pp. 299-306, 2015.
C. Kotanen, L. Martínez, R. Álvarez, J. Simecek, “Surface enhanced Raman scattering spectroscopy for detection and identification of microbial pathogens isolated from human serum”. Sens. Biosens. Res.; 8, pp. 20-26, 2016.
H. Zhou, D. Yang, N.P. Ivleva et al., “SERS Detection of Bacteria in Water by in Situ Coating with Ag Nanoparticles”, Anal Chem; 86, pp. 1525-1536, 2014.
D. Graham, K. Faulds, “Chemical Science Simultaneous detection and quanti fi cation of three bacterial meningitis pathogens by SERS”. Chem Sci; 5, pp. 1030-1040, 2014.
B. Allegranzi, Report on the Burden of Endemic Health Care-Associated Infection Worldwide Clean Care is Safer Care. WHO 2011, pp. 1-40.
Secretaría de Salud de Bogotá, Sistema de vigilancia epidemiológica de infecciones intrahospitalarias. Secretaria de Salud Distrital de Bogotá, 1984; pp. 1-35
K. Kneipp, H. Kneipp, B. Kartha et al., “Detection and identification of a single DNA base molecule using surface-enhanced Raman”, Phys Rev E; 57, pp. 6281-6290, 1998.
M. Culha, M. Kahraman, C. Dilek, “Rapid identification of bacteria and yeast using surface-enhanced Raman scattering”, Surf Interf Anal; 42, pp. 462-465, 2010.
H. Zhou, D. Yang, N. Ivleva, N. Mircescu, R. Niessner, C. Haish, “SERS Detection of Bacteria in Water by in Situ Coating with Ag Nanoparticles”, Anal Chem; 86, pp. 1525-1533, 2014.
R.M. Jarvis, R. Goodacre, “Discrimination of Bacteria Using Surface-Enhanced Raman Spectroscopy”, Anal Chem; 76, pp. 40-47, 2004.
G. McNay, D. Eustace, W.E. Smith et al., “Surface-enhanced Raman scattering (SERS) and surface-enhanced resonance Raman scattering (SERRS): a review of applications”, Appl Spectrosc; 65, pp. 825-37, 2011.
I.S. Patel, W.R. Premasiri, D.T. Moir, L.D. Ziegler, “Barcoding bacterial cells: a SERS-based methodology for pathogen identification”, J Raman Spect; 39, pp. 1660-1672, 2008.
Y. Wang, K. Lee, J. Irudayaraj, “Silver Nanosphere SERS Probes for Sensitive Identification of Pathogens”, J Phys Chem C; 114, pp. 16122-16128, 2010.
A.J. Driscoll, M.H. Harpster, P.A. Johnson, “The development of surface-enhanced Raman scattering as a detection modality for portable in vitro diagnostics: progress and challenges”, Phys Chem Phys Chem 2013; 15, pp. 20415-20433. doi: 10.1039/c3cp52334a
W.R. Premasiri, N. Krieger, W.R. Associates, “Characterization of the Surface Enhanced Raman Scattering (SERS) of Bacteria”, J Chem Phys B; 109, pp. 312-320, 2005.
J. Chen, X. Wu, Y. Huang, Y. Zhao, “Chemical Detection of E. coli using SERS active filters with silver nanorod array”, Sensors Actuators B Chem; 191, pp. 485-490, 2014
J.J. Castillo, T. Rindzevicius, K. Wu et al. “Silver-capped silicon nanopillar platforms for adsorption studies of folic acid using surface enhanced Raman spectroscopy and density functional theory”, J Raman Spect; 46, pp. 1087-1094, 2015.
J.J. Castillo, T. Rindzevicius et al., “Adsorption and Vibrational Study of Folic Acid on Gold Nanopillar Structures Using Surface-enhanced Raman Scattering Spectroscopy Regular Paper”, Nanomat Nanotechn; 5: 1-7, 2015.
J. Borrero, Y. Chen, G. Dunny, Y. Kaznessis, “Modified Lactic Acid Bacteria Detect and Inhibit Multiresistant Enterococci”, ACS Synthet. Biol.; 4, pp. 299-306, 2015.
C. Kotanen, L. Martínez, R. Álvarez, J. Simecek, “Surface enhanced Raman scattering spectroscopy for detection and identification of microbial pathogens isolated from human serum”. Sens. Biosens. Res.; 8, pp. 20-26, 2016.
H. Zhou, D. Yang, N.P. Ivleva et al., “SERS Detection of Bacteria in Water by in Situ Coating with Ag Nanoparticles”, Anal Chem; 86, pp. 1525-1536, 2014.
D. Graham, K. Faulds, “Chemical Science Simultaneous detection and quanti fi cation of three bacterial meningitis pathogens by SERS”. Chem Sci; 5, pp. 1030-1040, 2014.
B. Allegranzi, Report on the Burden of Endemic Health Care-Associated Infection Worldwide Clean Care is Safer Care. WHO 2011, pp. 1-40.
Secretaría de Salud de Bogotá, Sistema de vigilancia epidemiológica de infecciones intrahospitalarias. Secretaria de Salud Distrital de Bogotá, 1984; pp. 1-35
K. Kneipp, H. Kneipp, B. Kartha et al., “Detection and identification of a single DNA base molecule using surface-enhanced Raman”, Phys Rev E; 57, pp. 6281-6290, 1998.
M. Culha, M. Kahraman, C. Dilek, “Rapid identification of bacteria and yeast using surface-enhanced Raman scattering”, Surf Interf Anal; 42, pp. 462-465, 2010.
H. Zhou, D. Yang, N. Ivleva, N. Mircescu, R. Niessner, C. Haish, “SERS Detection of Bacteria in Water by in Situ Coating with Ag Nanoparticles”, Anal Chem; 86, pp. 1525-1533, 2014.
R.M. Jarvis, R. Goodacre, “Discrimination of Bacteria Using Surface-Enhanced Raman Spectroscopy”, Anal Chem; 76, pp. 40-47, 2004.
G. McNay, D. Eustace, W.E. Smith et al., “Surface-enhanced Raman scattering (SERS) and surface-enhanced resonance Raman scattering (SERRS): a review of applications”, Appl Spectrosc; 65, pp. 825-37, 2011.
I.S. Patel, W.R. Premasiri, D.T. Moir, L.D. Ziegler, “Barcoding bacterial cells: a SERS-based methodology for pathogen identification”, J Raman Spect; 39, pp. 1660-1672, 2008.
Y. Wang, K. Lee, J. Irudayaraj, “Silver Nanosphere SERS Probes for Sensitive Identification of Pathogens”, J Phys Chem C; 114, pp. 16122-16128, 2010.
How to Cite
Castillo-León, J., Rincón-Orozco, B., & Cabanzo-Hernández, R. (1). Silver nanostructured platforms for detecting Escherichia coli through Raman scattering spectroscopy “Proof of concept”. ITECKNE, 14(2), 164-169. https://doi.org/https://doi.org/10.15332/iteckne.v14i2.1771
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Research and Innovation Articles
